› 3vz3

X-ray diffraction
1.69Å resolution

Structural insights into substrate and cofactor selection by sp2771

Released:
DOI: 10.2210/pdb3vz3/pdb
PDB entry 3vz3 coloured by chain, front view.
PDB entry 3vz3 coloured by chain, side view.
PDB entry 3vz3 coloured by chain, top view.
Source organism: Synechococcus sp. PCC 7002
Primary publication:
Structural basis for cofactor and substrate selection by cyanobacterium succinic semialdehyde dehydrogenase.
Yuan Z, Yin B, Wei D, Yuan YR
J Struct Biol 182 125-35 (2013)
PMID: 23500184

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109429 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 457 amino acids
Theoretical weight: 49.31 KDa
Source organism: Synechococcus sp. PCC 7002
Expression system: Escherichia coli
UniProt:
  • Canonical: B1XMM6 (Residues: 1-454; Coverage: 100%)
Gene name: SYNPCC7002_A2771
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SSN 2 x SSN
Ligand NAP 2 x NAP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 42.957Å b: 117.181Å c: 181.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.203
Expression system: Escherichia coli

Quick links

Citations

10 citation in other articles

An evolutionary metabolic engineering approach for enhancing lipogenesis in Yarrowia lipolytica.
Liu et al. (2015)
9 more

2 mentions without citation

Kinetic and structural characterization for cofactor preference of succinic semialdehyde dehydrogenase from Streptococcus pyogenes.
Jang et al. (2014)
1 more