› 3vzh

X-ray diffraction
1.7Å resolution

Crystal Structure of CRISPR-associated Protein

Released:
DOI: 10.2210/pdb3vzh/pdb
PDB entry 3vzh coloured by chain, front view.
PDB entry 3vzh coloured by chain, side view.
PDB entry 3vzh coloured by chain, top view.
Source organism: Streptococcus pyogenes serotype M1
Primary publication:
Conservation and variability in the structure and function of the Cas5d endoribonuclease in the CRISPR-mediated microbial immune system.
Koo Y, Ka D, Kim EJ, Suh N, Bae E
J Mol Biol 425 3799-810 (2013)
PMID: 23500492

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-189417 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
pre-crRNA processing endonuclease Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 27.96 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99YS3 (Residues: 1-242; Coverage: 100%)
Gene name: SPy_1566
Sequence domains: CRISPR-associated protein (Cas_Cas5)
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6B
Spacegroup: C2
Unit cell:
a: 123.323Å b: 43.781Å c: 37.006Å
α: 90° β: 90.75° γ: 90°
R-values:
R R work R free
0.185 0.183 0.226
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Unravelling the structural and mechanistic basis of CRISPR-Cas systems.
van der Oost et al. (2014)
9 more

4 mentions without citation

Annotation and Classification of CRISPR-Cas Systems.
Makarova et al. (2015)
3 more