› 3vzk

X-ray diffraction
1.55Å resolution

Crystal structure of the Bacillus circulans endo-beta-(1,4)-xylanase (BcX) N35E mutant

Released:
DOI: 10.2210/pdb3vzk/pdb
PDB entry 3vzk coloured by chain, front view.
PDB entry 3vzk coloured by chain, side view.
PDB entry 3vzk coloured by chain, top view.
Source organism: Niallia circulans
Primary publication:
Strategies for modulating the pH-dependent activity of a family 11 glycoside hydrolase.
Ludwiczek ML, D'Angelo I, Yalloway GN, Brockerman JA, Okon M, Nielsen JE, Strynadka NC, Withers SG, McIntosh LP
Biochemistry 52 3138-56 (2013)
PMID: 23578322

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140770 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 185 amino acids
Theoretical weight: 20.42 KDa
Source organism: Niallia circulans
Expression system: Escherichia coli
UniProt:
  • Canonical: P09850 (Residues: 29-213; Coverage: 100%)
Gene name: xlnA
Sequence domains: Glycosyl hydrolases family 11
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 39.327Å b: 62.814Å c: 64.126Å
α: 90° β: 104.57° γ: 90°
R-values:
R R work R free
0.163 0.162 0.197
Expression system: Escherichia coli

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Citations

2 review citations

Classification, mode of action and production strategy of xylanase and its application for biofuel production from water hyacinth.
Uday et al. (2016)
1 more