› 3w08

X-ray diffraction
1.8Å resolution

Crystal structure of aldoxime dehydratase

Released:
DOI: 10.2210/pdb3w08/pdb
PDB entry 3w08 coloured by chain, front view.
PDB entry 3w08 coloured by chain, side view.
PDB entry 3w08 coloured by chain, top view.
Source organism: Pseudomonas chlororaphis
Primary publication:
Crystal structure of aldoxime dehydratase and its catalytic mechanism involved in carbon-nitrogen triple-bond synthesis.
Nomura J, Hashimoto H, Ohta T, Hashimoto Y, Wada K, Naruta Y, Oinuma K, Kobayashi M
Proc Natl Acad Sci U S A 110 2810-5 (2013)
PMID: 23382199

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182130 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aliphatic aldoxime dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 352 amino acids
Theoretical weight: 40.18 KDa
Source organism: Pseudomonas chlororaphis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7WSJ4 (Residues: 1-352; Coverage: 100%)
Gene name: oxdA
Sequence domains: Haem-containing dehydratase

Ligands and Environments

1 bound ligand:
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2
Unit cell:
a: 152.946Å b: 84.458Å c: 76.36Å
α: 90° β: 114.32° γ: 90°
R-values:
R R work R free
0.158 0.157 0.189
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Nitrile Metabolizing Enzymes in Biocatalysis and Biotransformation.
Bhalla et al. (2018)
1 more

2 mentions without citation

Substrate, product, and cofactor: The extraordinarily flexible relationship between the CDE superfamily and heme.
Celis et al. (2015)
1 more