3w3c

X-ray diffraction
2.43Å resolution

Crystal structure of VirB core domain complexed with the cis-acting site upstream icsb promoter

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-117069 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Virulence regulon transcriptional activator VirB Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.25 KDa
Source organism: Shigella flexneri 2a
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A247 (Residues: 129-250; Coverage: 40%)
Gene names: CP0123, invE, virB
Sequence domains: ParB family
Structure domains: Arc Repressor Mutant, subunit A
DNA (26-MER) Chain: B
Molecule details ›
Chain: B
Length: 26 nucleotides
Theoretical weight: 8.1 KDa
Source organism: Shigella flexneri 2a
Expression system: Not provided
DNA (26-MER) Chain: C
Molecule details ›
Chain: C
Length: 26 nucleotides
Theoretical weight: 7.88 KDa
Source organism: Shigella flexneri 2a
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 56.14Å b: 163.4Å c: 39.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.265 0.264 0.28
Expression systems:
  • Escherichia coli
  • Not provided