› 3w3d

X-ray diffraction
1.8Å resolution

Crystal structure of smooth muscle G actin DNase I complex

Released:
DOI: 10.2210/pdb3w3d/pdb
PDB entry 3w3d coloured by chain, front view.
PDB entry 3w3d coloured by chain, side view.
PDB entry 3w3d coloured by chain, top view.
Source organisms:
Primary publication:
Refined structure and solvent network of chicken gizzard G-actin DNase 1 complex at 1.8A resolution
Sasaki K, Sakabe K, Sakabe N, Kondo H, Shimomur M
Acta Crystallogr., A, Found. Crystallogr. 49 C111-C112 (1993)

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132782 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Actin, gamma-enteric smooth muscle Chain: A
Molecule details ›
Chain: A
Length: 374 amino acids
Theoretical weight: 41.73 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P63270 (Residues: 3-376; Coverage: 100%)
Gene names: ACTA3, ACTG2, ACTSG
Sequence domains: Actin
Structure domains:
Deoxyribonuclease-1 Chain: B
Molecule details ›
Chain: B
Length: 260 amino acids
Theoretical weight: 29.09 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00639 (Residues: 23-282; Coverage: 100%)
Gene names: DNASE1, DNL1
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand ATP 1 x ATP
Ligand CA 2 x CA
1 modified residue:
Ligand HIC 1 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 42Å b: 225.3Å c: 77.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 not available

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