› 3w53

X-ray diffraction
2.2Å resolution

Crystal structure of psychrophilic beta-glucosidase BglU from Micrococcus antarcticus

Released:
DOI: 10.2210/pdb3w53/pdb
PDB entry 3w53 coloured by chain, front view.
PDB entry 3w53 coloured by chain, side view.
PDB entry 3w53 coloured by chain, top view.
Source organism: Micrococcus antarcticus
Entry authors: Hou YJ, Miao LL, Li DF, Liu ZP

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110905 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase Chain: A
Molecule details ›
Chain: A
Length: 506 amino acids
Theoretical weight: 56.31 KDa
Source organism: Micrococcus antarcticus
Expression system: Escherichia coli
UniProt:
  • Canonical: B9V8P5 (Residues: 1-472; Coverage: 100%)
Gene name: bgl
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2221
Unit cell:
a: 57.28Å b: 84.67Å c: 184.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.161 0.2
Expression system: Escherichia coli

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