› 3w54

X-ray diffraction
2.3Å resolution

Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with colletochlorin B

Released:
DOI: 10.2210/pdb3w54/pdb
PDB entry 3w54 coloured by chain, front view.
PDB entry 3w54 coloured by chain, side view.
PDB entry 3w54 coloured by chain, top view.
Source organism: Trypanosoma brucei brucei
Primary publication:
Structure of the trypanosome cyanide-insensitive alternative oxidase.
Shiba T, Kido Y, Sakamoto K, Inaoka DK, Tsuge C, Tatsumi R, Takahashi G, Balogun EO, Nara T, Aoki T, Honma T, Tanaka A, Inoue M, Matsuoka S, Saimoto H, Moore AL, Harada S, Kita K
Proc Natl Acad Sci U S A 110 4580-5 (2013)
PMID: 23487766

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173253 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alternative oxidase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 329 amino acids
Theoretical weight: 37.64 KDa
Source organism: Trypanosoma brucei brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q26710 (Residues: 1-329; Coverage: 100%)
Gene name: AOX
Sequence domains: Alternative oxidase
Structure domains: Alternative oxidase

Ligands and Environments

5 bound ligands:
Ligand FE 8 x FE
Ligand OH 4 x OH
Ligand RNB 4 x RNB
Ligand SO4 2 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 228.017Å b: 137.92Å c: 63.058Å
α: 90° β: 106.08° γ: 90°
R-values:
R R work R free
0.188 0.185 0.227
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Alternative oxidase: a mitochondrial respiratory pathway to maintain metabolic and signaling homeostasis during abiotic and biotic stress in plants.
Vanlerberghe GC. (2013)
13 more

6 mentions without citation

Structure of the trypanosome cyanide-insensitive alternative oxidase.
Shiba et al. (2013)
5 more