› 3w5b

X-ray diffraction
3.2Å resolution

Crystal structure of the recombinant SERCA1a (calcium pump of fast twitch skeletal muscle) in the E1.Mg2+ state

Released:
DOI: 10.2210/pdb3w5b/pdb
PDB entry 3w5b coloured by chain, front view.
PDB entry 3w5b coloured by chain, side view.
PDB entry 3w5b coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Crystal structures of the calcium pump and sarcolipin in the Mg2+-bound E1 state.
Toyoshima C, Iwasawa S, Ogawa H, Hirata A, Tsueda J, Inesi G
Nature 495 260-4 (2013)
PMID: 23455422

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Chain: A
Molecule details ›
Chain: A
Length: 1000 amino acids
Theoretical weight: 110.17 KDa
Source organism: Oryctolagus cuniculus
Expression system: Chlorocebus sabaeus
UniProt:
  • Canonical: P04191 (Residues: 1-993; Coverage: 99%)
Gene name: ATP2A1
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NA 1 x NA
Ligand MG 2 x MG
Ligand TM1 1 x TM1
Ligand PTY 4 x PTY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 186.787Å b: 55.293Å c: 178.278Å
α: 90° β: 118.18° γ: 90°
R-values:
R R work R free
0.269 0.268 0.298
Expression system: Chlorocebus sabaeus

Quick links

Citations

22 review citations

Ca(2+) homeostasis and endoplasmic reticulum (ER) stress: An integrated view of calcium signaling.
Krebs et al. (2015)
21 more

12 mentions without citation

Primary Active Ca2+ Transport Systems in Health and Disease.
Chen et al. (2020)
11 more