› 3w5g

X-ray diffraction
3Å resolution

Crystal structure of tomato beta-galactosidase 4 in complex with galactose

Released:
DOI: 10.2210/pdb3w5g/pdb
PDB entry 3w5g coloured by chain, front view.
PDB entry 3w5g coloured by chain, side view.
PDB entry 3w5g coloured by chain, top view.
Source organism: Solanum lycopersicum
Entry authors: Eda M, Tada T

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131337 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-galactosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 718 amino acids
Theoretical weight: 79.78 KDa
Source organism: Solanum lycopersicum
Expression system: Komagataella pastoris
UniProt:
  • Canonical: O81100 (Residues: 24-724; Coverage: 100%)
Gene name: TBG4
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 3 x NAG
Ligand GAL 8 x GAL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 93.93Å b: 106.382Å c: 162.379Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.2 0.259
Expression system: Komagataella pastoris

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