› 3w68

X-ray diffraction
2.05Å resolution

Crystal structure of mouse alpha-tocopherol transfer protein in complex with alpha-tocopherol and phosphatidylinositol-(4,5)-bisphosphate

Released:
DOI: 10.2210/pdb3w68/pdb
PDB entry 3w68 coloured by chain, front view.
PDB entry 3w68 coloured by chain, side view.
PDB entry 3w68 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Impaired α-TTP-PIPs interaction underlies familial vitamin E deficiency.
Kono N, Ohto U, Hiramatsu T, Urabe M, Uchida Y, Satow Y, Arai H
Science 340 1106-10 (2013)
PMID: 23599266

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-183917 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-tocopherol transfer protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 266 amino acids
Theoretical weight: 30.62 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8BWP5 (Residues: 21-278; Coverage: 93%)
Gene name: Ttpa
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand VIV 4 x VIV
Ligand 4PT 3 x 4PT
Ligand PBU 1 x PBU
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P1
Unit cell:
a: 57.977Å b: 69.62Å c: 87.097Å
α: 100.88° β: 109.45° γ: 100.01°
R-values:
R R work R free
0.201 0.199 0.231
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Polyphosphoinositide binding domains: Key to inositol lipid biology.
Hammond et al. (2015)
21 more

3 mentions without citation

Interaction of Fapp1 with Arf1 and PI4P at a membrane surface: an example of coincidence detection.
Liu et al. (2014)
2 more