› 3w6s

X-ray diffraction
1.9Å resolution

yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance via proline metabolism

Released:
DOI: 10.2210/pdb3w6s/pdb
PDB entry 3w6s coloured by chain, front view.
PDB entry 3w6s coloured by chain, side view.
PDB entry 3w6s coloured by chain, top view.
Source organism: Saccharomyces cerevisiae Sigma1278b
Primary publication:
Structural and functional analysis of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance via proline metabolism.
Nasuno R, Hirano Y, Itoh T, Hakoshima T, Hibi T, Takagi H
Proc Natl Acad Sci U S A 110 11821-6 (2013)
PMID: 23818613

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + L-glutamate-5-semialdehyde = CoA + N-acetyl-L-glutamate 5-semialdehyde
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyltransferase MPR1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 243 amino acids
Theoretical weight: 27.94 KDa
Source organism: Saccharomyces cerevisiae Sigma1278b
Expression system: Escherichia coli
UniProt:
  • Canonical: E9P8D2 (Residues: 1-229; Coverage: 100%)
Gene name: MPR1
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments

2 bound ligands:
Ligand P6G 3 x P6G
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P3112
Unit cell:
a: 83.862Å b: 83.862Å c: 193.819Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.166 0.187
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Role of Proline in Pathogen and Host Interactions.
Christgen et al. (2019)
2 more

2 mentions without citation

Structure and Functional Diversity of GCN5-Related N-Acetyltransferases (GNAT).
Salah Ud-Din et al. (2016)
1 more