› 3w7v

X-ray diffraction
1.85Å resolution

Crystal Structure of Axe2, an Acetylxylan Esterase from Geobacillus stearothermophilus

Released:
DOI: 10.2210/pdb3w7v/pdb
PDB entry 3w7v coloured by chain, front view.
PDB entry 3w7v coloured by chain, side view.
PDB entry 3w7v coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
A unique octameric structure of Axe2, an intracellular acetyl-xylooligosaccharide esterase from Geobacillus stearothermophilus.
Lansky S, Alalouf O, Solomon HV, Alhassid A, Govada L, Chayen NE, Belrhali H, Shoham Y, Shoham G
Acta Crystallogr D Biol Crystallogr 70 261-78 (2014)
PMID: 24531461

Function and Biology Details

Reaction catalysed: 
Deacetylation of xylans and xylo-oligosaccharides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetylxylan esterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 24.8 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09LX1 (Residues: 1-219; Coverage: 100%)
Gene name: axe2
Sequence domains: GDSL-like Lipase/Acylhydrolase family
Structure domains: SGNH hydrolase

Ligands and Environments

2 bound ligands:
Ligand GOL 5 x GOL
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: I422
Unit cell:
a: 109.832Å b: 109.832Å c: 213.308Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.141 0.177
Expression system: Escherichia coli

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Citations

1 review citation

Understanding the structural and functional properties of carbohydrate esterases with a special focus on hemicellulose deacetylating acetyl xylan esterases.
Sista Kameshwar et al. (2018)
9 other articles

6 mentions without citation

Structural and chemical biology of deacetylases for carbohydrates, proteins, small molecules and histones.
Bürger et al. (2018)
5 more