› 3w9b

X-ray diffraction
2.9Å resolution

Crystal structure of Candida antarctica lipase B with anion-tag

Released:
DOI: 10.2210/pdb3w9b/pdb
PDB entry 3w9b coloured by chain, front view.
PDB entry 3w9b coloured by chain, side view.
PDB entry 3w9b coloured by chain, top view.
Source organism: Moesziomyces antarcticus
Entry authors: Kim SK, Lee HH, Park YC, Jeon ST, Son SH, Min WK, Seo JH

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154387 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase B Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 328 amino acids
Theoretical weight: 34.27 KDa
Source organism: Moesziomyces antarcticus
Expression system: Escherichia coli
UniProt:
  • Canonical: P41365 (Residues: 26-342; Coverage: 98%)
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand PE8 4 x PE8
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P65
Unit cell:
a: 123.659Å b: 123.659Å c: 191.852Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.232 0.265
Expression system: Escherichia coli

Quick links