› 3wai

X-ray diffraction
1.9Å resolution

Crystal structure of the C-terminal globular domain of oligosaccharyltransferase (AfAglB-L, O29867_ARCFU) from Archaeoglobus fulgidus as a MBP fusion

Released:
DOI: 10.2210/pdb3wai/pdb
PDB entry 3wai coloured by chain, front view.
PDB entry 3wai coloured by chain, side view.
PDB entry 3wai coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the C-terminal globular domain of the third paralog of the Archaeoglobus fulgidus oligosaccharyltransferases.
Matsumoto S, Shimada A, Kohda D
BMC Struct Biol 13 11 (2013)
PMID: 23815857

Function and Biology Details

Reaction catalysed: 
An archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128176 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dolichyl-phosphooligosaccharide-protein glycotransferase 3; Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 739 amino acids
Theoretical weight: 82.97 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: O29867 (Residues: 500-868; Coverage: 43%)
Gene names: AF_0380, JW3994, aglB3, b4034, malE
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 180.239Å b: 52.93Å c: 78.418Å
α: 90° β: 90.71° γ: 90°
R-values:
R R work R free
0.166 0.164 0.202
Expression system: Escherichia coli

Quick links

Citations

4 review citations

N-linked glycosylation in Archaea: a structural, functional, and genetic analysis.
Jarrell et al. (2014)
3 more

7 mentions without citation

Structural Insight into the Mechanism of N-Linked Glycosylation by Oligosaccharyltransferase.
Mohanty et al. (2020)
6 more