› 3waj

X-ray diffraction
2.5Å resolution

Crystal structure of the Archaeoglobus fulgidus oligosaccharyltransferase (O29867_ARCFU) complex with Zn and sulfate

Released:
DOI: 10.2210/pdb3waj/pdb
PDB entry 3waj coloured by chain, front view.
PDB entry 3waj coloured by chain, side view.
PDB entry 3waj coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Crystal structures of an archaeal oligosaccharyltransferase provide insights into the catalytic cycle of N-linked protein glycosylation.
Matsumoto S, Shimada A, Nyirenda J, Igura M, Kawano Y, Kohda D
Proc Natl Acad Sci U S A 110 17868-73 (2013)
PMID: 24127570

Function and Biology Details

Reaction catalysed: 
An archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128177 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dolichyl-phosphooligosaccharide-protein glycotransferase 3 Chain: A
Molecule details ›
Chain: A
Length: 875 amino acids
Theoretical weight: 99.11 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O29867 (Residues: 1-868; Coverage: 100%)
Gene names: AF_0380, aglB3
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 234.195Å b: 108.961Å c: 56.069Å
α: 90° β: 96.08° γ: 90°
R-values:
R R work R free
0.184 0.182 0.216
Expression system: Escherichia coli

Quick links

Citations

14 review citations

N-linked glycosylation in Archaea: a structural, functional, and genetic analysis.
Jarrell et al. (2014)
13 more

12 mentions without citation

Advances in understanding glycosyltransferases from a structural perspective.
Gloster TM. (2014)
11 more