› 3wak

X-ray diffraction
3.41Å resolution

Crystal structure of the Archaeoglobus fulgidus oligosaccharyltransferase (O29867_ARCFU) in the apo form

Released:
DOI: 10.2210/pdb3wak/pdb
PDB entry 3wak coloured by chain, front view.
PDB entry 3wak coloured by chain, side view.
PDB entry 3wak coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Crystal structures of an archaeal oligosaccharyltransferase provide insights into the catalytic cycle of N-linked protein glycosylation.
Matsumoto S, Shimada A, Nyirenda J, Igura M, Kawano Y, Kohda D
Proc Natl Acad Sci U S A 110 17868-73 (2013)
PMID: 24127570

Function and Biology Details

Reaction catalysed: 
An archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128177 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dolichyl-phosphooligosaccharide-protein glycotransferase 3 Chain: A
Molecule details ›
Chain: A
Length: 875 amino acids
Theoretical weight: 99.11 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O29867 (Residues: 1-868; Coverage: 100%)
Gene names: AF_0380, aglB3
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P43212
Unit cell:
a: 123.357Å b: 123.357Å c: 182.496Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.272
Expression system: Escherichia coli

Quick links

Citations

18 review citations

N-linked glycosylation in Archaea: a structural, functional, and genetic analysis.
Jarrell et al. (2014)
17 more

7 mentions without citation

Structural Insight into the Mechanism of N-Linked Glycosylation by Oligosaccharyltransferase.
Mohanty et al. (2020)
6 more