› 3wb0

X-ray diffraction
1.91Å resolution

HcgB from Methanocaldococcus jannaschii in complex with light-decomposed FeGP cofactor of [Fe]-hydrogenase

Released:
DOI: 10.2210/pdb3wb0/pdb
PDB entry 3wb0 coloured by chain, front view.
PDB entry 3wb0 coloured by chain, side view.
PDB entry 3wb0 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
Identification of the HcgB enzyme in [Fe]-hydrogenase-cofactor biosynthesis.
Fujishiro T, Tamura H, Schick M, Kahnt J, Xie X, Ermler U, Shima S
Angew Chem Int Ed Engl 52 12555-8 (2013)
PMID: 24249552

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176500 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized protein MJ0488 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 166 amino acids
Theoretical weight: 18.87 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57912 (Residues: 3-158; Coverage: 99%)
Gene name: MJ0488
Sequence domains: FeGP cofactor biosynthesis protein HcgB, guanylyltransferase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FEG 4 x FEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 52.96Å b: 119.34Å c: 55.65Å
α: 90° β: 115.14° γ: 90°
R-values:
R R work R free
0.229 0.227 0.275
Expression system: Escherichia coli

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Citations

2 review citations

Radical S-adenosyl-L-methionine chemistry in the synthesis of hydrogenase and nitrogenase metal cofactors.
Byer et al. (2015)
1 more