› 3wbn

X-ray diffraction
2.45Å resolution

Crystal structure of MATE in complex with MaL6

Released:
DOI: 10.2210/pdb3wbn/pdb
PDB entry 3wbn coloured by chain, front view.
PDB entry 3wbn coloured by chain, side view.
PDB entry 3wbn coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Structural basis for the drug extrusion mechanism by a MATE multidrug transporter.
Tanaka Y, Hipolito CJ, Maturana AD, Ito K, Kuroda T, Higuchi T, Katoh T, Kato HE, Hattori M, Kumazaki K, Tsukazaki T, Ishitani R, Suga H, Nureki O
Nature 496 247-51 (2013)
PMID: 23535598

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164204 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
MATE family efflux transporter Chain: A
Molecule details ›
Chain: A
Length: 461 amino acids
Theoretical weight: 49.28 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U2X0 (Residues: 1-461; Coverage: 100%)
Gene name: PF0708
Sequence domains: MatE
MaL6 Chain: B
Molecule details ›
Chain: B
Length: 19 amino acids
Theoretical weight: 2.3 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand OLC 5 x OLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: C2
Unit cell:
a: 152.728Å b: 59.828Å c: 68.034Å
α: 90° β: 91.34° γ: 90°
R-values:
R R work R free
0.19 0.186 0.227
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

51 review citations

The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.
Li et al. (2015)
50 more

8 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
7 more