› 3wbz

X-ray diffraction
2.39Å resolution

Crystal structure of C. albicans tRNA(His) guanylyltransferase (Thg1) with ATP

Released:
DOI: 10.2210/pdb3wbz/pdb
PDB entry 3wbz coloured by chain, front view.
PDB entry 3wbz coloured by chain, side view.
PDB entry 3wbz coloured by chain, top view.
Source organism: Candida albicans SC5314
Primary publication:
Structural basis of reverse nucleotide polymerization.
Nakamura A, Nemoto T, Heinemann IU, Yamashita K, Sonoda T, Komoda K, Tanaka I, Söll D, Yao M
Proc Natl Acad Sci U S A 110 20970-5 (2013)
PMID: 24324136

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164205 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Likely histidyl tRNA-specific guanylyltransferase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 271 amino acids
Theoretical weight: 32.69 KDa
Source organism: Candida albicans SC5314
Expression system: Escherichia coli
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand ATP 16 x ATP
Ligand MG 24 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 74.83Å b: 217.87Å c: 87.24Å
α: 90° β: 113.68° γ: 90°
R-values:
R R work R free
0.177 0.176 0.208
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Mitochondrial transcription and translation: overview.
D'Souza et al. (2018)
4 more

3 mentions without citation

Structural basis of reverse nucleotide polymerization.
Nakamura et al. (2013)
2 more