› 3wc2

X-ray diffraction
3.64Å resolution

Crystal structure of C. albicans tRNA(His) guanylyltransferase (Thg1) with a tRNA(Phe)(GUG)

Released:
DOI: 10.2210/pdb3wc2/pdb
PDB entry 3wc2 coloured by chain, front view.
PDB entry 3wc2 coloured by chain, side view.
PDB entry 3wc2 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of reverse nucleotide polymerization.
Nakamura A, Nemoto T, Heinemann IU, Yamashita K, Sonoda T, Komoda K, Tanaka I, Söll D, Yao M
Proc Natl Acad Sci U S A 110 20970-5 (2013)
PMID: 24324136

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-164209 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Likely histidyl tRNA-specific guanylyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 271 amino acids
Theoretical weight: 32.69 KDa
Source organism: Candida albicans SC5314
Expression system: Escherichia coli
Structure domains: Alpha-Beta Plaits
76mer-tRNA Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 76 nucleotides
Theoretical weight: 24.51 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P3121
Unit cell:
a: 96.74Å b: 96.74Å c: 299.36Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.255 0.253 0.288
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Mitochondrial transcription and translation: overview.
D'Souza et al. (2018)
4 more

4 mentions without citation

Structural basis of reverse nucleotide polymerization.
Nakamura et al. (2013)
3 more