› 3wck

X-ray diffraction
2.3Å resolution

Crystal structure of monomeric photosensitizing fluorescent protein, Supernova

Released:
DOI: 10.2210/pdb3wck/pdb
PDB entry 3wck coloured by chain, front view.
PDB entry 3wck coloured by chain, side view.
PDB entry 3wck coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation.
Takemoto K, Matsuda T, Sakai N, Fu D, Noda M, Uchiyama S, Kotera I, Arai Y, Horiuchi M, Fukui K, Ayabe T, Inagaki F, Suzuki H, Nagai T
Sci Rep 3 2629 (2013)
PMID: 24043132

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174037 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
KillerRed Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 271 amino acids
Theoretical weight: 30.35 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2TCH5 (Residues: 2-237; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRQ 8 x CRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P1
Unit cell:
a: 60.252Å b: 89.304Å c: 109.885Å
α: 70.57° β: 89.95° γ: 71.62°
R-values:
R R work R free
0.184 0.181 0.234
Expression system: Escherichia coli

Quick links

Citations

33 review citations

Reactive oxygen species generating systems meeting challenges of photodynamic cancer therapy.
Zhou et al. (2016)
32 more

2 mentions without citation

Photodynamic Physiology-Photonanomanipulations in Cellular Physiology with Protein Photosensitizers.
Jiang et al. (2017)
1 more