› 3wdu

X-ray diffraction
2.25Å resolution

The complex structure of PtLic16A with cellobiose

Released:
DOI: 10.2210/pdb3wdu/pdb
PDB entry 3wdu coloured by chain, front view.
PDB entry 3wdu coloured by chain, side view.
PDB entry 3wdu coloured by chain, top view.
Source organism: Paecilomyces sp. 'thermophila'
Primary publication:
Structural and mutagenetic analyses of a 1,3-1,4-β-glucanase from Paecilomyces thermophila.
Cheng YS, Huang CH, Chen CC, Huang TY, Ko TP, Huang JW, Wu TH, Liu JR, Guo RT
Biochim Biophys Acta 1844 366-73 (2014)
PMID: 24262091

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122728 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
GH16 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 297 amino acids
Theoretical weight: 32.1 KDa
Source organism: Paecilomyces sp. 'thermophila'
Expression system: Komagataella pastoris
UniProt:
  • Canonical: E0XN39 (Residues: 19-314; Coverage: 100%)
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
1 bound ligand:
Ligand BGC 2 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: C2221
Unit cell:
a: 88.142Å b: 172.559Å c: 166.376Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.213
Expression system: Komagataella pastoris

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Citations

1 review citation

The features that distinguish lichenases from other polysaccharide-hydrolyzing enzymes and the relevance of lichenases for biotechnological applications.
Goldenkova-Pavlova et al. (2018)
8 other articles