› 3wfo

X-ray diffraction
3.4Å resolution

tRNA processing enzyme (apo form 1)

Released:
DOI: 10.2210/pdb3wfo/pdb
PDB entry 3wfo coloured by chain, front view.
PDB entry 3wfo coloured by chain, side view.
PDB entry 3wfo coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Translocation and rotation of tRNA during template-independent RNA polymerization by tRNA nucleotidyltransferase.
Yamashita S, Takeshita D, Tomita K
Structure 22 315-25 (2014)
PMID: 24389024

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130582 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CC-adding tRNA nucleotidyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 525 amino acids
Theoretical weight: 60.53 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67911 (Residues: 1-512; Coverage: 100%)
Gene name: aq_2158
Sequence domains:
Structure domains: Beta Polymerase, domain 2

Ligands and Environments

1 bound ligand:
Ligand SO4 11 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C222
Unit cell:
a: 177.481Å b: 327.769Å c: 78.102Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.21
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The tRNA Elbow in Structure, Recognition and Evolution.
Zhang et al. (2016)
5 more