› 3wfp

X-ray diffraction
4Å resolution

tRNA processing enzyme (apo form 2)

Released:
DOI: 10.2210/pdb3wfp/pdb
PDB entry 3wfp coloured by chain, front view.
PDB entry 3wfp coloured by chain, side view.
PDB entry 3wfp coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Translocation and rotation of tRNA during template-independent RNA polymerization by tRNA nucleotidyltransferase.
Yamashita S, Takeshita D, Tomita K
Structure 22 315-25 (2014)
PMID: 24389024

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130582 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CC-adding tRNA nucleotidyltransferase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 497 amino acids
Theoretical weight: 55.83 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
UniProt:
  • Canonical: O67911 (Residues: 17-450; Coverage: 85%)
Gene name: aq_2158
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P21
Unit cell:
a: 126.91Å b: 138.36Å c: 147.62Å
α: 90° β: 111.04° γ: 90°
R-values:
R R work R free
0.26 0.258 0.314
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The tRNA Elbow in Structure, Recognition and Evolution.
Zhang et al. (2016)
5 more

2 mentions without citation

NMR reveals structural rearrangements associated to substrate insertion in nucleotide-adding enzymes.
Mohanty et al. (2016)
1 more