3wfr

X-ray diffraction
3.5Å resolution

tRNA processing enzyme complex 2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-130583 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
CC-adding tRNA nucleotidyltransferase Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 512 amino acids
Theoretical weight: 57.15 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
UniProt:
  • Canonical: O67911 (Residues: 16-453; Coverage: 86%)
Gene name: aq_2158
Sequence domains:
Structure domains: Beta Polymerase, domain 2
RNA (74-MER) Chains: A, B
Molecule details ›
Chains: A, B
Length: 74 nucleotides
Theoretical weight: 23.85 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Not provided
Sequence domains: tRNA
RNA (75-MER) Chains: C, D
Molecule details ›
Chains: C, D
Length: 75 nucleotides
Theoretical weight: 24.16 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P212121
Unit cell:
a: 152.81Å b: 154.59Å c: 174.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.224 0.269
Expression systems:
  • Escherichia coli
  • Not provided