› 3wg1

X-ray diffraction
1.9Å resolution

Crystal structure of Agrocybe cylindracea galectin with lactose

Released:
DOI: 10.2210/pdb3wg1/pdb
PDB entry 3wg1 coloured by chain, front view.
PDB entry 3wg1 coloured by chain, side view.
PDB entry 3wg1 coloured by chain, top view.
Source organism: Cyclocybe aegerita
Primary publication:
Conformational change of a unique sequence in a fungal galectin from Agrocybe cylindracea controls glycan ligand-binding specificity.
Kuwabara N, Hu D, Tateno H, Makyio H, Hirabayashi J, Kato R
FEBS Lett 587 3620-5 (2013)
PMID: 24036446

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Anti-tumor lectin Chains: A, B
Molecule details ›
Chains: A, B
Length: 178 amino acids
Theoretical weight: 19 KDa
Source organism: Cyclocybe aegerita
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6WY08 (Residues: 1-158; Coverage: 100%)
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P61
Unit cell:
a: 125.368Å b: 125.368Å c: 56.589Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.173 0.196
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Mushroom lectins: specificity, structure and bioactivity relevant to human disease.
Hassan et al. (2015)
4 more