› 3wg4

X-ray diffraction
1.6Å resolution

Crystal structure of Agrocybe cylindracea galectin mutant (N46A) with blood type A antigen tetraose

Released:
DOI: 10.2210/pdb3wg4/pdb
PDB entry 3wg4 coloured by chain, front view.
PDB entry 3wg4 coloured by chain, side view.
PDB entry 3wg4 coloured by chain, top view.
Source organism: Agrocybe cylindracea
Primary publication:
Conformational change of a unique sequence in a fungal galectin from Agrocybe cylindracea controls glycan ligand-binding specificity.
Kuwabara N, Hu D, Tateno H, Makyio H, Hirabayashi J, Kato R
FEBS Lett 587 3620-5 (2013)
PMID: 24036446

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Anti-tumor lectin Chains: A, B
Molecule details ›
Chains: A, B
Length: 178 amino acids
Theoretical weight: 18.96 KDa
Source organism: Agrocybe cylindracea
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6WY08 (Residues: 1-158; Coverage: 100%)
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GAL, FUC, A2G
Carbohydrate polymer
1 bound ligand:
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P65
Unit cell:
a: 102.824Å b: 102.824Å c: 74.578Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.149 0.148 0.175
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Entomotoxic and nematotoxic lectins and protease inhibitors from fungal fruiting bodies.
Sabotič et al. (2016)
3 more

1 mention without citation

Mushroom lectins: specificity, structure and bioactivity relevant to human disease.
Hassan et al. (2015)