› 3wh1

X-ray diffraction
1Å resolution

Crystal Structure of a Family GH19 Chitinase from Bryum coronatum in complex with (GlcNAc)4 at 1.0 A resolution

Released:
DOI: 10.2210/pdb3wh1/pdb
PDB entry 3wh1 coloured by chain, front view.
PDB entry 3wh1 coloured by chain, side view.
PDB entry 3wh1 coloured by chain, top view.
Source organism: Gemmabryum coronatum
Primary publication:
Crystal structure of a "loopless" GH19 chitinase in complex with chitin tetrasaccharide spanning the catalytic center.
Ohnuma T, Umemoto N, Nagata T, Shinya S, Numata T, Taira T, Fukamizo T
Biochim Biophys Acta 1844 793-802 (2014)
PMID: 24582745

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109103 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glycoside hydrolase family 19 catalytic domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 22.74 KDa
Source organism: Gemmabryum coronatum
Expression system: Escherichia coli
UniProt:
  • Canonical: A9ZSX9 (Residues: 24-228; Coverage: 100%)
Gene name: bcchiA
Sequence domains: Chitinase class I
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2
Unit cell:
a: 74.384Å b: 58.375Å c: 48.136Å
α: 90° β: 115.47° γ: 90°
R-values:
R R work R free
0.148 0.148 0.155
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Interaction between chitosan and its related enzymes: A review.
Shinya et al. (2017)
1 more

11 mentions without citation

Insights into the structure and function of membrane-integrated processive glycosyltransferases.
Bi et al. (2015)
10 more