› 3whc

X-ray diffraction
2.2Å resolution

Crystal structure of a transcriptional regulator FadR from Bacillus subtilis in complex with stearoyl-CoA

Released:
DOI: 10.2210/pdb3whc/pdb
PDB entry 3whc coloured by chain, front view.
PDB entry 3whc coloured by chain, side view.
PDB entry 3whc coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Structural characterization of a ligand-bound form of Bacillus subtilis FadR involved in the regulation of fatty acid degradation.
Fujihashi M, Nakatani T, Hirooka K, Matsuoka H, Fujita Y, Miki K
Proteins 82 1301-10 (2014)
PMID: 24356978

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161118 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fatty acid metabolism regulator protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 194 amino acids
Theoretical weight: 22.01 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P94548 (Residues: 1-194; Coverage: 100%)
Gene names: BSU28550, fadR, ysiA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ST9 6 x ST9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 70.305Å b: 88.762Å c: 199.952Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.239 0.271
Expression system: Escherichia coli

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Citations

2 review citations

Role of fatty acids in Bacillus environmental adaptation.
Diomandé et al. (2015)
1 more

1 mention without citation

Structural basis of operator sites recognition and effector binding in the TetR family transcription regulator FadR.
Yeo et al. (2017)