› 3whe

X-ray diffraction
4Å resolution

A new conserved neutralizing epitope at the globular head of hemagglutinin in H3N2 influenza viruses

Released:
DOI: 10.2210/pdb3whe/pdb
PDB entry 3whe coloured by chain, front view.
PDB entry 3whe coloured by chain, side view.
PDB entry 3whe coloured by chain, top view.
Source organisms:
Primary publication:
Conserved neutralizing epitope at globular head of hemagglutinin in H3N2 influenza viruses.
Iba Y, Fujii Y, Ohshima N, Sumida T, Kubota-Koketsu R, Ikeda M, Wakiyama M, Shirouzu M, Okada J, Okuno Y, Kurosawa Y, Yokoyama S
J Virol 88 7130-44 (2014)
PMID: 24719430

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235422 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (6 distinct):
Hemagglutinin Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 493 amino acids
Theoretical weight: 55.22 KDa
Source organism: Influenza A virus (A/Aichi/2-1/1968(H3N2))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: T2HNI1 (Residues: 25-517; Coverage: 87%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
immunoglobulin heavy chain Chains: 1, 3, 5, 7, 9, M, O, Q, S, U, W, Y
Molecule details ›
Chains: 1, 3, 5, 7, 9, M, O, Q, S, U, W, Y
Length: 226 amino acids
Theoretical weight: 24.19 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
immunoglobulin light chain Chains: 0, 2, 4, 6, 8, N, P, R, T, V, X, Z
Molecule details ›
Chains: 0, 2, 4, 6, 8, N, P, R, T, V, X, Z
Length: 220 amino acids
Theoretical weight: 22.94 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand NAG 24 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 391.037Å b: 241.173Å c: 223.214Å
α: 90° β: 123.62° γ: 90°
R-values:
R R work R free
0.236 0.235 0.308
Expression systems:
  • Spodoptera frugiperda
  • Cricetulus griseus

Quick links

Citations

18 review citations

From Original Antigenic Sin to the Universal Influenza Virus Vaccine.
Henry et al. (2018)
17 more

11 mentions without citation

Antibody specific epitope prediction-emergence of a new paradigm.
Sela-Culang et al. (2015)
10 more