› 3whj

X-ray diffraction
1.65Å resolution

Crystal structure of Nas2 N-terminal domain

Released:
DOI: 10.2210/pdb3whj/pdb
PDB entry 3whj coloured by chain, front view.
PDB entry 3whj coloured by chain, side view.
PDB entry 3whj coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural basis for proteasome formation controlled by an assembly chaperone nas2.
Satoh T, Saeki Y, Hiromoto T, Wang YH, Uekusa Y, Yagi H, Yoshihara H, Yagi-Utsumi M, Mizushima T, Tanaka K, Kato K
Structure 22 731-43 (2014)
PMID: 24685148

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-154142 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable 26S proteasome regulatory subunit p27 Chain: A
Molecule details ›
Chain: A
Length: 122 amino acids
Theoretical weight: 13.86 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P40555 (Residues: 1-120; Coverage: 55%)
Gene names: NAS2, YIL007C
Sequence domains: Nas2 N_terminal domain
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand CD 5 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P6122
Unit cell:
a: 64.59Å b: 64.59Å c: 149.76Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.159 0.197
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Proteasome Structure and Assembly.
Budenholzer et al. (2017)
5 more

1 mention without citation

An Aspergillus nidulans β-mannanase with high transglycosylation capacity revealed through comparative studies within glycosidase family 5.
Rosengren et al. (2014)