› 3whl

X-ray diffraction
4Å resolution

Crystal structure of Nas2 N-terminal domain complexed with PAN-Rpt5C chimera

Released:
DOI: 10.2210/pdb3whl/pdb
PDB entry 3whl coloured by chain, front view.
PDB entry 3whl coloured by chain, side view.
PDB entry 3whl coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for proteasome formation controlled by an assembly chaperone nas2.
Satoh T, Saeki Y, Hiromoto T, Wang YH, Uekusa Y, Yagi H, Yoshihara H, Yagi-Utsumi M, Mizushima T, Tanaka K, Kato K
Structure 22 731-43 (2014)
PMID: 24685148

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152573 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
26S proteasome regulatory subunit 6A; Proteasome-activating nucleotidase Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 270 amino acids
Theoretical weight: 30.02 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U4H3 (Residues: 125-309; Coverage: 47%)
  • Canonical: P33297 (Residues: 356-434; Coverage: 18%)
Gene names: O3258, PF0115, RPT5, YOR117W, YOR3258W, YTA1, pan
Sequence domains:
Structure domains:
Probable 26S proteasome regulatory subunit p27 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 122 amino acids
Theoretical weight: 13.86 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P40555 (Residues: 1-120; Coverage: 55%)
Gene names: NAS2, YIL007C
Sequence domains: Nas2 N_terminal domain
Structure domains: Helix Hairpins

Ligands and Environments

1 bound ligand:
Ligand ATP 4 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P3121
Unit cell:
a: 110.63Å b: 110.63Å c: 251.94Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.233 0.285
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Regulation of proteasome assembly and activity in health and disease.
Rousseau et al. (2018)
3 more

2 mentions without citation

Proteasome Structure and Assembly.
Budenholzer et al. (2017)
1 more