› 3wi5

X-ray diffraction
2.4Å resolution

Crystal structure of the Loop 7 mutant PorB from Neisseria meningitidis serogroup B

Released:
DOI: 10.2210/pdb3wi5/pdb
PDB entry 3wi5 coloured by chain, front view.
PDB entry 3wi5 coloured by chain, side view.
PDB entry 3wi5 coloured by chain, top view.
Source organism: Neisseria meningitidis
Primary publication:
Crystallographic analysis of Neisseria meningitidis PorB extracellular loops potentially implicated in TLR2 recognition.
Kattner C, Toussi DN, Zaucha J, Wetzler LM, Rüppel N, Zachariae U, Massari P, Tanabe M
J Struct Biol 185 440-7 (2014)
PMID: 24361688

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151789 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major outer membrane protein P.IB Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 33.83 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli
UniProt:
  • Canonical: P30690 (Residues: 20-331; Coverage: 100%)
Gene names: NMB2039, porB
Sequence domains: Gram-negative porin
Structure domains: Porin

Ligands and Environments

1 bound ligand:
Ligand FLC 2 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: R32
Unit cell:
a: 143.486Å b: 143.486Å c: 178.42Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.227 0.256
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Immune Adjuvant Effect of Molecularly-defined Toll-Like Receptor Ligands.
Toussi et al. (2014)
2 more

1 mention without citation

Crystallographic analysis of Neisseria meningitidis PorB extracellular loops potentially implicated in TLR2 recognition.
Kattner et al. (2014)