› 3wii

X-ray diffraction
1.6Å resolution

Crystal structure of the Fab fragment of B2212A, a murine monoclonal antibody specific for the third fibronectin domain (Fn3) of human ROBO1.

Released:
DOI: 10.2210/pdb3wii/pdb
PDB entry 3wii coloured by chain, front view.
PDB entry 3wii coloured by chain, side view.
PDB entry 3wii coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural features of interfacial tyrosine residue in ROBO1 fibronectin domain-antibody complex: Crystallographic, thermodynamic, and molecular dynamic analyses.
Nakayama T, Mizohata E, Yamashita T, Nagatoishi S, Nakakido M, Iwanari H, Mochizuki Y, Kado Y, Yokota Y, Satoh R, Tsumoto K, Fujitani H, Kodama T, Hamakubo T, Inoue T
Protein Sci 24 328-40 (2015)
PMID: 25492858

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213437 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
anti-human ROBO1 antibody B2212A Fab light chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 213 amino acids
Theoretical weight: 23.57 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
anti-human ROBO1 antibody B2212A Fab heavy chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 220 amino acids
Theoretical weight: 23.8 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 41.823Å b: 136.49Å c: 77.512Å
α: 90° β: 91.86° γ: 90°
R-values:
R R work R free
0.187 0.185 0.23

Quick links

Citations

2 review citations

Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins.
Hanashima et al. (2021)
1 more

3 mentions without citation

Transferring entropy to the realm of GxG interactions.
Ferrario et al. (2018)
2 more