› 3wip

X-ray diffraction
2.6Å resolution

Crystal structure of acetylcholine bound to Ls-AChBP

Released:
DOI: 10.2210/pdb3wip/pdb
PDB entry 3wip coloured by chain, front view.
PDB entry 3wip coloured by chain, side view.
PDB entry 3wip coloured by chain, top view.
Source organism: Lymnaea stagnalis
Primary publication:
Molecular recognition of the neurotransmitter acetylcholine by an acetylcholine binding protein reveals determinants of binding to nicotinic acetylcholine receptors.
Olsen JA, Balle T, Gajhede M, Ahring PK, Kastrup JS
PLoS One 9 e91232 (2014)
PMID: 24637639

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157653 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetylcholine-binding protein Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 229 amino acids
Theoretical weight: 26.09 KDa
Source organism: Lymnaea stagnalis
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P58154 (Residues: 1-229; Coverage: 100%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

5 bound ligands:
Ligand ACH 10 x ACH
Ligand 1PE 12 x 1PE
Ligand SO4 7 x SO4
Ligand ACT 3 x ACT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: C2
Unit cell:
a: 236.85Å b: 73.15Å c: 132.47Å
α: 90° β: 101.45° γ: 90°
R-values:
R R work R free
0.182 0.179 0.239
Expression system: Spodoptera frugiperda

Quick links

Citations

5 review citations

Nicotinic acetylcholine receptor inhibitors derived from snake and snail venoms.
Dutertre et al. (2017)
4 more

17 mentions without citation

Understanding structure-function relationships of the human neuronal acetylcholine receptor: insights from the first crystal structures of neuronal subunits.
Giastas et al. (2018)
16 more