› 3wja

X-ray diffraction
2.55Å resolution

The crystal structure of human cytosolic NADP(+)-dependent malic enzyme in apo form

Released:
DOI: 10.2210/pdb3wja/pdb
PDB entry 3wja coloured by chain, front view.
PDB entry 3wja coloured by chain, side view.
PDB entry 3wja coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural characteristics of the nonallosteric human cytosolic malic enzyme.
Hsieh JY, Li SY, Chen MC, Yang PC, Chen HY, Chan NL, Liu JH, Hung HC
Biochim Biophys Acta 1844 1773-83 (2014)
PMID: 24998673

Function and Biology Details

Reaction catalysed: 
(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155711 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent malic enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 580 amino acids
Theoretical weight: 65.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P48163 (Residues: 1-572; Coverage: 100%)
Gene name: ME1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Unit cell:
a: 70.213Å b: 116.138Å c: 182.403Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.222
Expression system: Escherichia coli

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Citations

1 review citation

6-Phosphogluconate dehydrogenase and its crystal structures.
Hanau et al. (2022)
5 other articles

7 mentions without citation

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