› 3wjj

X-ray diffraction
2.6Å resolution

Crystal structure of IIb selective Fc variant, Fc(P238D), in complex with FcgRIIb

Released:
DOI: 10.2210/pdb3wjj/pdb
PDB entry 3wjj coloured by chain, front view.
PDB entry 3wjj coloured by chain, side view.
PDB entry 3wjj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Engineered antibody Fc variant with selectively enhanced FcγRIIb binding over both FcγRIIa(R131) and FcγRIIa(H131).
Mimoto F, Katada H, Kadono S, Igawa T, Kuramochi T, Muraoka M, Wada Y, Haraya K, Miyazaki T, Hattori K
Protein Eng Des Sel 26 589-98 (2013)
PMID: 23744091

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134859 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin heavy constant gamma 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 25.94 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01857 (Residues: 99-328; Coverage: 58%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Low affinity immunoglobulin gamma Fc region receptor II-b Chain: C
Molecule details ›
Chain: C
Length: 179 amino acids
Theoretical weight: 20.26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P31994 (Residues: 45-217; Coverage: 65%)
Gene names: CD32, FCG2, FCGR2B, IGFR2
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL, FUL
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P21
Unit cell:
a: 48.85Å b: 76.01Å c: 115.08Å
α: 90° β: 100.7° γ: 90°
R-values:
R R work R free
0.232 0.23 0.272
Expression system: Homo sapiens

Quick links

Citations

35 review citations

Mouse and human FcR effector functions.
Bruhns et al. (2015)
34 more

8 mentions without citation

IgG Fc engineering to modulate antibody effector functions.
Wang et al. (2018)
7 more