› 3wla

X-ray diffraction
1.9Å resolution

Crystal Structure of sOPH Native

Released:
DOI: 10.2210/pdb3wla/pdb
PDB entry 3wla coloured by chain, front view.
PDB entry 3wla coloured by chain, side view.
PDB entry 3wla coloured by chain, top view.
Source organism: Sphingopyxis sp. 113P3
Primary publication:
Structural insights into enzymatic degradation of oxidized polyvinyl alcohol.
Yang Y, Ko TP, Liu L, Li J, Huang CH, Chan HC, Ren F, Jia D, Wang AH, Guo RT, Chen J, Du G
Chembiochem 15 1882-6 (2014)
PMID: 25044912

Function and Biology Details

Reaction catalysed: 
Nonane-4,6-dione + H(2)O = pentan-2-one + butanoate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176708 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxidized polyvinyl alcohol hydrolase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 336 amino acids
Theoretical weight: 36.54 KDa
Source organism: Sphingopyxis sp. 113P3
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q588Z2 (Residues: 35-364; Coverage: 100%)
Gene name: oph
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P1
Unit cell:
a: 58.34Å b: 75.742Å c: 75.98Å
α: 74.04° β: 88.67° γ: 74.01°
R-values:
R R work R free
0.195 0.193 0.239
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

Biodegradable Polymeric Materials in Degradable Electronic Devices.
Feig et al. (2018)
1 more

1 mention without citation

Crystal Structure and Substrate Specificity Modification of Acetyl Xylan Esterase from Aspergillus luchuensis.
Komiya et al. (2017)