› 3wme

X-ray diffraction
2.75Å resolution

Crystal structure of an inward-facing eukaryotic ABC multidrug transporter

Released:
DOI: 10.2210/pdb3wme/pdb
PDB entry 3wme coloured by chain, front view.
PDB entry 3wme coloured by chain, side view.
PDB entry 3wme coloured by chain, top view.
Source organism: Cyanidioschyzon merolae strain 10D
Primary publication:
Structural basis for gating mechanisms of a eukaryotic P-glycoprotein homolog.
Kodan A, Yamaguchi T, Nakatsu T, Sakiyama K, Hipolito CJ, Fujioka A, Hirokane R, Ikeguchi K, Watanabe B, Hiratake J, Kimura Y, Suga H, Ueda K, Kato H
Proc Natl Acad Sci U S A 111 4049-54 (2014)
PMID: 24591620

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-125963 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable ATP-dependent transporter ycf16 Chain: A
Molecule details ›
Chain: A
Length: 612 amino acids
Theoretical weight: 66.13 KDa
Source organism: Cyanidioschyzon merolae strain 10D
Expression system: Komagataella pastoris
UniProt:
  • Canonical: M1VAN7 (Residues: 93-696; Coverage: 87%)
Gene name: CYME_CMD148C
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand DMU 1 x DMU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: R32
Unit cell:
a: 180.312Å b: 180.312Å c: 152.332Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.209 0.275
Expression system: Komagataella pastoris

Quick links

Citations

30 review citations

Mechanistic diversity in ATP-binding cassette (ABC) transporters.
Locher KP. (2016)
29 more

8 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
7 more