› 3wmt

X-ray diffraction
1.5Å resolution

Crystal structure of feruloyl esterase B from Aspergillus oryzae

Released:
DOI: 10.2210/pdb3wmt/pdb
PDB entry 3wmt coloured by chain, front view.
PDB entry 3wmt coloured by chain, side view.
PDB entry 3wmt coloured by chain, top view.
Source organism: Aspergillus oryzae RIB40
Primary publication:
Crystal structure of a feruloyl esterase belonging to the tannase family: a disulfide bond near a catalytic triad.
Suzuki K, Hori A, Kawamoto K, Thangudu RR, Ishida T, Igarashi K, Samejima M, Yamada C, Arakawa T, Wakagi T, Koseki T, Fushinobu S
Proteins 82 2857-67 (2014)
PMID: 25066066

Function and Biology Details

Reaction catalysed: 
Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174076 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable feruloyl esterase B-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 522 amino acids
Theoretical weight: 56.62 KDa
Source organism: Aspergillus oryzae RIB40
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q2UP89 (Residues: 19-540; Coverage: 100%)
Gene names: AO090001000066, faeB-1
Sequence domains: Tannase and feruloyl esterase

Ligands and Environments

2 bound ligands:
Ligand NAG 8 x NAG
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21212
Unit cell:
a: 93.954Å b: 142.937Å c: 73.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.141 0.168
Expression system: Komagataella pastoris

Quick links

Citations

5 review citations

Diversity of fungal feruloyl esterases: updated phylogenetic classification, properties, and industrial applications.
Dilokpimol et al. (2016)
4 more

10 mentions without citation

Carboxylic ester hydrolases: Classification and database derived from their primary, secondary, and tertiary structures.
Chen et al. (2016)
9 more