› 3wnb

X-ray diffraction
1.7Å resolution

Crystal structure of EF-Pyl in complex with GMPPNP

Released:
DOI: 10.2210/pdb3wnb/pdb
PDB entry 3wnb coloured by chain, front view.
PDB entry 3wnb coloured by chain, side view.
PDB entry 3wnb coloured by chain, top view.
Source organism: Methanosarcina mazei
Primary publication:
A SelB/EF-Tu/aIF2γ-like protein from Methanosarcina mazei in the GTP-bound form binds cysteinyl-tRNA(Cys.).
Yanagisawa T, Ishii R, Hikida Y, Fukunaga R, Sengoku T, Sekine S, Yokoyama S
J Struct Funct Genomics 16 25-41 (2015)
PMID: 25618148

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185820 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein translation elongation factor 1A Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 40.77 KDa
Source organism: Methanosarcina mazei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8PXB3 (Residues: 1-350; Coverage: 100%)
Gene name: MM_1309
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GNP 1 x GNP
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21212
Unit cell:
a: 62.061Å b: 108.703Å c: 58.315Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.189 0.233
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

A SelB/EF-Tu/aIF2γ-like protein from Methanosarcina mazei in the GTP-bound form binds cysteinyl-tRNA(Cys.).
Yanagisawa et al. (2015)