› 3wno

X-ray diffraction
1.9Å resolution

D308A mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with cycloisomaltooctaose

Released:
DOI: 10.2210/pdb3wno/pdb
PDB entry 3wno coloured by chain, front view.
PDB entry 3wno coloured by chain, side view.
PDB entry 3wno coloured by chain, top view.
Source organism: Niallia circulans
Primary publication:
Structural elucidation of the cyclization mechanism of α-1,6-glucan by Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase.
Suzuki N, Fujimoto Z, Kim YM, Momma M, Kishine N, Suzuki R, Suzuki S, Kitamura S, Kobayashi M, Kimura A, Funane K
J Biol Chem 289 12040-12051 (2014)
PMID: 24616103

Function and Biology Details

Reaction catalysed: 
Cyclizes part of a (1->6)-alpha-D-glucan chain by formation of a (1->6)-alpha-D-glucosidic bond
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161097 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Cycloisomaltooligosaccharide glucanotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 710 amino acids
Theoretical weight: 79.13 KDa
Source organism: Niallia circulans
Expression system: Escherichia coli
UniProt:
  • Canonical: P94286 (Residues: 39-738; Coverage: 75%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
5 bound ligands:
Ligand CA 2 x CA
Ligand NA 2 x NA
Ligand SO4 13 x SO4
Ligand EDO 9 x EDO
Ligand MES 2 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 61.928Å b: 167.715Å c: 174.861Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.195
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Using structure to inform carbohydrate binding module function.
Abbott et al. (2014)
2 more

3 mentions without citation

Structural analysis of glucuronoxylan-specific Xyn30D and its attached CBM35 domain gives insights into the role of modularity in specificity.
Sainz-Polo et al. (2014)
2 more