› 3wo7

X-ray diffraction
3.2Å resolution

Crystal structure of YidC from Bacillus halodurans (form II)

Released:
DOI: 10.2210/pdb3wo7/pdb
PDB entry 3wo7 coloured by chain, front view.
PDB entry 3wo7 coloured by chain, side view.
PDB entry 3wo7 coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Primary publication:
Structural basis of Sec-independent membrane protein insertion by YidC.
Kumazaki K, Chiba S, Takemoto M, Furukawa A, Nishiyama K, Sugano Y, Mori T, Dohmae N, Hirata K, Nakada-Nakura Y, Maturana AD, Tanaka Y, Mori H, Sugita Y, Arisaka F, Ito K, Ishitani R, Tsukazaki T, Nureki O
Nature 509 516-20 (2014)
PMID: 24739968

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191846 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Membrane protein insertase YidC 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 254 amino acids
Theoretical weight: 29.29 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KDP2 (Residues: 27-267; Coverage: 93%)
Gene names: BH1169, yidC2
Sequence domains: 60Kd inner membrane protein

Ligands and Environments

1 bound ligand:
Ligand CU 4 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P21
Unit cell:
a: 54.033Å b: 70.058Å c: 82.91Å
α: 90° β: 92.05° γ: 90°
R-values:
R R work R free
0.263 0.26 0.291
Expression system: Escherichia coli

Quick links

Citations

38 review citations

Mechanisms of integral membrane protein insertion and folding.
Cymer et al. (2015)
37 more

8 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
7 more