› 3woh

X-ray diffraction
2.5Å resolution

Structure of Ketoreductase SiaM from Streptomyces sp. A7248

Released:
DOI: 10.2210/pdb3woh/pdb
PDB entry 3woh coloured by chain, front view.
PDB entry 3woh coloured by chain, side view.
PDB entry 3woh coloured by chain, top view.
Source organism: Streptomyces sp. A7248
Primary publication:
Structural insight into the tetramerization of an iterative ketoreductase siam through aromatic residues in the interfaces.
Wang H, Zhang H, Zou Y, Mi Y, Lin S, Xie Z, Yan Y, Zhang H
PLoS One 9 e97996 (2014)
PMID: 24901639

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125657 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] reductase Chain: A
Molecule details ›
Chain: A
Length: 251 amino acids
Theoretical weight: 26.08 KDa
Source organism: Streptomyces sp. A7248
Expression system: Escherichia coli
UniProt:
  • Canonical: K9M8L2 (Residues: 2-239; Coverage: 100%)
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P42212
Unit cell:
a: 56.425Å b: 56.425Å c: 122.838Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.271
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Ketide Synthase (KS) Domain Prediction and Analysis of Iterative Type II PKS Gene in Marine Sponge-Associated Actinobacteria Producing Biosurfactants and Antimicrobial Agents.
Selvin et al. (2016)
2 more

2 mentions without citation

The Uncommon Enzymology of Cis-Acyltransferase Assembly Lines.
Keatinge-Clay AT. (2017)
1 more