› 3wp9

X-ray diffraction
1.6Å resolution

Crystal structure of antifreeze protein from an Antarctic sea ice bacterium Colwellia sp.

Released:
DOI: 10.2210/pdb3wp9/pdb
PDB entry 3wp9 coloured by chain, front view.
PDB entry 3wp9 coloured by chain, side view.
PDB entry 3wp9 coloured by chain, top view.
Source organism: Colwellia sp. SLW05
Primary publication:
Hyperactive antifreeze protein from an Antarctic sea ice bacterium Colwellia sp. has a compound ice-binding site without repetitive sequences.
Hanada Y, Nishimiya Y, Miura A, Tsuda S, Kondo H
FEBS J 281 3576-90 (2014)
PMID: 24938370

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107777 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ice-binding protein Chain: A
Molecule details ›
Chain: A
Length: 226 amino acids
Theoretical weight: 23.5 KDa
Source organism: Colwellia sp. SLW05
Expression system: Escherichia coli
UniProt:
  • Canonical: A5XB26 (Residues: 28-253; Coverage: 100%)
Sequence domains: Ice-binding-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2221
Unit cell:
a: 90.25Å b: 106.95Å c: 44.075Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.242
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Microbial ecology of the cryosphere: sea ice and glacial habitats.
Boetius et al. (2015)
7 more

10 mentions without citation

Ice Binding Proteins: Diverse Biological Roles and Applications in Different Types of Industry.
Białkowska et al. (2020)
9 more