› 3wpe

X-ray diffraction
2.38Å resolution

Crystal structure of bovine TLR9 in complex with agonistic DNA1668_12mer

Released:
DOI: 10.2210/pdb3wpe/pdb
PDB entry 3wpe coloured by chain, front view.
PDB entry 3wpe coloured by chain, side view.
PDB entry 3wpe coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of CpG and inhibitory DNA recognition by Toll-like receptor 9.
Ohto U, Shibata T, Tanji H, Ishida H, Krayukhina E, Uchiyama S, Miyake K, Shimizu T
Nature 520 702-5 (2015)
PMID: 25686612

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117082 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Toll-like receptor 9 Chain: A
Molecule details ›
Chain: A
Length: 801 amino acids
Theoretical weight: 89.32 KDa
Source organism: Bos taurus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q5I2M5 (Residues: 25-815; Coverage: 79%)
Gene name: TLR9
Sequence domains:
Structure domains: Ribonuclease Inhibitor
DNA (5'-D(*CP*AP*TP*GP*AP*CP*GP*TP*TP*CP*CP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.61 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 115.82Å b: 113.08Å c: 67.43Å
α: 90° β: 100.61° γ: 90°
R-values:
R R work R free
0.22 0.217 0.274
Expression systems:
  • Drosophila melanogaster
  • Not provided

Quick links

Citations

64 review citations

Toll-like Receptors and the Control of Immunity.
Fitzgerald et al. (2020)
63 more

5 mentions without citation

Structural and functional understanding of the toll-like receptors.
Asami et al. (2021)
4 more