› 3wr2

X-ray diffraction
1.75Å resolution

RNase Po1 complexed with 3'GMP

Released:
DOI: 10.2210/pdb3wr2/pdb
Model geometry
Fit model/data
PDB entry 3wr2 coloured by chain, front view.
PDB entry 3wr2 coloured by chain, side view.
PDB entry 3wr2 coloured by chain, top view.
Source organism: Pleurotus ostreatus
Entry authors: Hara Y, Katsutani T, Kobayashi H, Suzuki M

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease Po1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 101 amino acids
Theoretical weight: 10.73 KDa
Source organism: Pleurotus ostreatus
Expression system: Escherichia coli
UniProt:
  • Canonical: P81762 (Residues: 1-101; Coverage: 100%)
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

1 bound ligand:
Ligand 3GP 6 x 3GP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P212121
Unit cell:
a: 55.072Å b: 95.866Å c: 106.225Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.211
Expression system: Escherichia coli

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