› 3wr7

X-ray diffraction
2.5Å resolution

Crystal Structure of Spermidine Acetyltransferase from Escherichia coli

Released:
DOI: 10.2210/pdb3wr7/pdb
PDB entry 3wr7 coloured by chain, front view.
PDB entry 3wr7 coloured by chain, side view.
PDB entry 3wr7 coloured by chain, top view.
Source organism: Escherichia coli LY180
Primary publication:
Molecular mechanism underlying promiscuous polyamine recognition by spermidine acetyltransferase.
Sugiyama S, Ishikawa S, Tomitori H, Niiyama M, Hirose M, Miyazaki Y, Higashi K, Murata M, Adachi H, Takano K, Murakami S, Inoue T, Mori Y, Kashiwagi K, Igarashi K, Matsumura H
Int J Biochem Cell Biol 76 87-97 (2016)
PMID: 27163532

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102384 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyltransferase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 170 amino acids
Theoretical weight: 20.3 KDa
Source organism: Escherichia coli LY180
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M3KKU5 (Residues: 1-170; Coverage: 100%)
Sequence domains: Acetyltransferase (GNAT) domain
Structure domains: Aminopeptidase

Ligands and Environments

2 bound ligands:
Ligand SPD 4 x SPD
Ligand COA 4 x COA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P23
Unit cell:
a: 148.688Å b: 148.688Å c: 148.688Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.276
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Effects of polyamines on protein synthesis and growth of Escherichia coli.
Igarashi et al. (2018)
4 other articles

4 mentions without citation

A Fusion Protein Based on the Second Subunit of Hemagglutinin of Influenza A/H2N2 Viruses Provides Cross Immunity.
Stepanova et al. (2016)
3 more