› 3wvc

X-ray diffraction
2Å resolution

Guanylylpyridinol (GP)-bound HcgF from Methanocaldococcus jannaschii

Released:
DOI: 10.2210/pdb3wvc/pdb
PDB entry 3wvc coloured by chain, front view.
PDB entry 3wvc coloured by chain, side view.
PDB entry 3wvc coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
Protein-pyridinol thioester precursor for biosynthesis of the organometallic acyl-iron ligand in [Fe]-hydrogenase cofactor.
Fujishiro T, Kahnt J, Ermler U, Shima S
Nat Commun 6 6895 (2015)
PMID: 25882909

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176670 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UPF0254 protein MJ1251 Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 19.25 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58649 (Residues: 1-167; Coverage: 100%)
Gene name: MJ1251
Sequence domains: FeGP cofactor biosynthesis protein HcgF

Ligands and Environments

3 bound ligands:
Ligand SO4 6 x SO4
Ligand ZGP 1 x ZGP
Ligand FEG 1 x FEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 39.57Å b: 97.53Å c: 47.12Å
α: 90° β: 111.17° γ: 90°
R-values:
R R work R free
0.166 0.163 0.23
Expression system: Escherichia coli

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Citations

3 review citations

Hydrogenase Enzymes and Their Synthetic Models: The Role of Metal Hydrides.
Schilter et al. (2016)
2 more